Effect of percutaneous and open tenotomy on continuity of tendoachillis in relapsed clubfoot

Background: Tenotomy of tendon Achilles is one of the major components of Ponseti method and necessary to complete correction in about 80 to 90 % of patients, it is safely procedure can be done in outpatient office as percutaneous technique under local anesthesia or in operative room under general anesthesia as mini-open technique. This study was designed to evaluate effect of tendoachillis tenotomy either percutaneous or open on continuity of tendoachillis by open evaluation in relapsed club foot. Patient & method: This study was conducted on 18 individuals. They were divided into 2 equal groups: Group I: containing 9 patients both sex had previously percutaneous tenotomy is done and Group II: 9 patients both sex had previously open tenotomy is done. Results: percutaneous technique found easy dissection in 6 patients (66.7%) and difficult dissection in 3 patients (33.3%) and continuity found central and regular contour in 7 patients (77.8%) and fibrotic mass in 2 patients (22.2%), open technique found easy dissection in 5 patients (55.6%) and difficult dissection in 4 patients (44.4%) and continuity found central and regular contour in 5 patients (55.6%) and fibrotic mass in 4 patients (44.4%), Conclusion: the techniques tenotomy of tendoachillis had no effect on continuity of tendoachillis either open or closed techniques in management of club foot

Allelopathic Effect of Cell-Free Medium of Microcystis aeruginosa Kützing on the Chromosomal Changes in Allium cepa Root Tips and Plumule Formation of Zea mays Seedling.

This work confirm the allelopathic toxic effect of both cell-free medium in log and death phase of growth of Microcystis aeruginosa on chromosomal changes of Allium cepa root tips and the plumule formation of Zea mays seedlings. The results showed high significant inhibition of mitotic index in Allium cepa root tips in addition, chromosomal abnormalities were also detected. There were morphological change occurred in the plumule formation of Zea mays seedlings. The results revealed that, seed soaked in log phase cell-free medium had less inhibitory effect on plumule formation than that the seed soaked in death phase cell-free medium of M. aeruginosa. This was confirmed by the allelopathic effect of toxins released during death phase of growth was more than log phase.